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  #1921  
Old 09.02.2020, 21:53
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Re: Ask a Scientist

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I'll save you the joke of asking what happens on that toilet, or jokes about the diet, and suggest you double check the fastener itself if it keeps getting loose. Here's the reason: if you tightened with a tool (not by hand), and there's already a spring washer in place, only 1 of 2 things is going on:

- Option 1: there's something lubricating the washer/bolt interface (either it's oily for whatever reason, or the cleaning fluid used at home, etc). The bolt stays in place because the friction between the bolt head and the washer is higher than the tension applied on the thread. If something is lubricating that, then the bolt will tend to slip.
- Option 2: the base material is giving. You tighen the fastener, it "sinks" into the base material, and it becomes loose.
There is a plastic washer at the porcelain, then comes a steal washer, then the bolt nut. No humidity there I would say.
According to what you say I could also try to roughen/sand the steal washer and it might work?

Not much to joke about. It doesn't come all lose but it starts to move slightly after a while and I'm a perfectionist.
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  #1922  
Old 09.02.2020, 22:06
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Re: Ask a Scientist

Could a condensation make it damp? Say the porcelain bowl is cold and curley sits on it for a good while reading or posting...heating it?
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  #1923  
Old 09.02.2020, 22:15
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Re: Ask a Scientist

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Could a condensation make it damp? Say the porcelain bowl is cold and curley sits on it for a good while reading or posting...heating it?

No.

You mean dripping sweat when I see groans in EF?
No.
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  #1924  
Old 10.02.2020, 07:47
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Re: Ask a Scientist

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No.

You mean dripping sweat when I see groans in EF?
No.
You really should consider getting a new toilet

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  #1925  
Old 10.02.2020, 13:55
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Re: Ask a Scientist

Hi curley, where do the toilet seat and the porcelain bowl fit into the order of hardware that you gave? From the top down, toilet seat > plastic washer > porcelain > split washer > nut? If so, be VERY careful tightening the nut with your spanner. Too much torque forcing metal on porcelain and you can crack the porcelain. It's one reason why you see those plastic wing nuts on toilet seats, so they are hand-tightened only.

I suggest putting a silicone washer between the porcelain and the split washer. Tighten till good and firm but don't keep tightening it as hard as you can!

By the way, are you sure the thread of the bolt isn't stripped or blocked? Does the bolt head turn when you turn the nut? Is it just one bolt that comes loose, or both?

If you bring your toilet bowl and seat over here to me, with the bolts, I'll sort it out for you.

Last edited by Guest; 10.02.2020 at 14:56. Reason: Correcting the hardware hierarchy
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  #1926  
Old 10.02.2020, 14:31
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Re: Ask a Scientist

Quote:
Hi curley, where do the toilet seat and the porcelain bowl fit into the order of hardware that you gave? From the top down, plastic washer > toilet seat > porcelain > split washer > nut? If so, be VERY careful tightening the nut with your spanner. Too much torque forcing metal on porcelain and you can crack the porcelain. It's one reason why you see those plastic wing nuts on toilet seats, so they are hand-tightened only.

I suggest putting a silicone washer between the porcelain and the split washer. Tighten till good and firm but don't keep tightening it as hard as you can!

By the way, are you sure the thread of the bolt isn't stripped or blocked? Does the bolt head turn when you turn the nut? Is it just one bolt that comes loose, or both?

If you bring your toilet bowl and seat over here to me, with the bolts, I'll sort it out for you.
Do remember to flush the toilet bowl before transporting
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  #1927  
Old 10.02.2020, 14:43
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Re: Ask a Scientist

Please post photos
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  #1928  
Old 10.02.2020, 16:42
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Re: Ask a Scientist

Quote:
Hi curley, where do the toilet seat and the porcelain bowl fit into the order of hardware that you gave? From the top down, toilet seat > plastic washer > porcelain > split washer > nut? If so, be VERY careful tightening the nut with your spanner. Too much torque forcing metal on porcelain and you can crack the porcelain. It's one reason why you see those plastic wing nuts on toilet seats, so they are hand-tightened only.

I suggest putting a silicone washer between the porcelain and the split washer. Tighten till good and firm but don't keep tightening it as hard as you can!

By the way, are you sure the thread of the bolt isn't stripped or blocked? Does the bolt head turn when you turn the nut? Is it just one bolt that comes loose, or both?

If you bring your toilet bowl and seat over here to me, with the bolts, I'll sort it out for you.
Ahh, that's good of you. You don't mind if I might bring some construction rubble as well, as it is wall mounted?
From the top down, toilet seat > plastic washer > porcelain > plastic washer >split washer > nut.
Yes I'm sure the thread of the bolt isn't stripped or blocked.
What bolt head? There is none, the last bit is the nut (you wouldn't get a headed bolt through the holes in the porcelain, never saw that either).
I get your point about not screwing it too tightly. Maybe some wing bolts (like former seats had) will do the trick by being better to tighten by hand without over doing it.


@omtatsat, you already posted the photo of my toilet, which I think violated my rights re privacy law. I will check on that, once I can comfortably sit on the toilet doing the research.
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  #1929  
Old 10.02.2020, 18:11
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Re: Ask a Scientist

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Yeap. Spanner in the UK, I think, and wrench in the US (or vice-versa?).
Yes, a wrench.

Not that I ever used it. Maybe on a bike, or sumfin.
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  #1930  
Old 14.02.2020, 20:45
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Re: Ask a Scientist

Should we be emptying bottles of wine in our bomb shelter?

NASA has confirmed that a 1Km wide asteroid will miss the planet by a few million miles Saturday.

NASA’s Center for Near-Earth Object Studies identified the asteroid as 163373 (2002 PZ39).
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  #1931  
Old 01.03.2020, 16:15
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Re: Ask a Scientist

A couple more random questions...

How can a stunt plane fly upside down? I vaguely recall that wings are aerofoils(sp?) where airflow provides lift on the lower surface. How does that work however when a plane is flying upside down? Or, is it more of a case that a brick would fly upside down if you attached a powerful enough engine to it...

How does a beercan 'widget' work?
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  #1932  
Old 01.03.2020, 18:46
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Re: Ask a Scientist

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A couple more random questions...

How can a stunt plane fly upside down? I vaguely recall that wings are aerofoils(sp?) where airflow provides lift on the lower surface. How does that work however when a plane is flying upside down? Or, is it more of a case that a brick would fly upside down if you attached a powerful enough engine to it...

How does a beercan 'widget' work?
I'm not a scientist, but I always thought that those stunt planes, with enough up (or down) flap and speed could for a time fly upside down.

Re beer, I'm not a scientist (again) but I do work with beer. It's because as the can is opened all the pressure within the can escapes immediately. But the small amount of beer (and therefore pressure) within the plastic "widgit" - escapes from the tiny hole therein more slowly and in a squirt.

Like beer poured from hand pumps in the north of England, if the "sparkler", the ring on the end of the tap, is tightened up, the beer has to rush through more quickly, hence creating a more creamy, less gassy pint and with a thicker head.

Yet another reason why beer in Yorkshire is the best. Not that we need more reasons of course.
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  #1933  
Old 01.03.2020, 21:05
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Re: Ask a Scientist

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I'm not a scientist, but I always thought that those stunt planes, with enough up (or down) flap and speed could for a time fly upside down.
The pilots angle their planes slightly nose-up while inverted so that the air running over the wings still generate the lift in the right direction. Also, stunt planes have curved upper and lower surfaces of their wings which means they're less fuel-efficient but able to fly equally well either way up. Regular wings are flatter on the bottom than the top, generating more lift when up the right way.

The biggest problem with most planes is that they're designed to use gravity to help with several systems in the engine - they need to be specially designed to operate inverted for more than about half a minute, or even less with some.
One example is the fuel tank - normally, the fuel is taken from the bottom of the tank ... aerobatic planes have a special system that uses gravity to tell where the 'bottom' of the tank is at any time, and take fuel from there.
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  #1934  
Old 02.03.2020, 06:38
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Re: Ask a Scientist

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Wingnuts (as we call them in the USA) are more easily tightened by hand, whereas traditional nuts need a wrench. I don't think they hold any better or worse though.
One's nuts will hold better with a spring or a serrated washer underneath, otherwise they will need constant attention
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  #1935  
Old 02.03.2020, 07:12
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Re: Ask a Scientist

Monday morning, nothing to do but browse forum threads
Has anyone experience with vaporfly shoes ? Are they as good as the hype? Why are the other companies not coming up with me too products?
If I adapt my old running shoes with a piece of foam and tin plate insert or attach a piece of box-spring mattress to the soles would I be banned from competitive racing?
https://www.wired.com/story/the-scie...marathon-shoe/
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  #1936  
Old 02.04.2020, 20:26
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Re: Ask a Scientist

Biology. My daughter needs to explain the assay procedure for the viability live/dead assay - as outlined in this document https://www.abcam.com/ps/products/11...0(website).pdf

There is talk of dilutions, but we don't recognise the notation. Can anyone explain what 1X and 2X mean in:

Label cells with the Live and Dead Dye
Add 1X Live and Dead Dye to cells
(e.g. mix 2X dye in PBS 1:1 with cells in culture media )
Incubate 10 min at room temperature

And in this:
Dilute Live and Dead Dye to 2X concentration in PBS. E.g. 1mL PBS + 2µL 1000X Live and DeadDye.
how the "e.g." relates to the first sentence...
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  #1937  
Old 02.04.2020, 20:49
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Re: Ask a Scientist

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There is talk of dilutions, but we don't recognise the notation. Can anyone explain what 1X and 2X mean in:

Label cells with the Live and Dead Dye
Add 1X Live and Dead Dye to cells
(e.g. mix 2X dye in PBS 1:1 with cells in culture media )
Incubate 10 min at room temperature

And in this:
Dilute Live and Dead Dye to 2X concentration in PBS. E.g. 1mL PBS + 2µL 1000X Live and DeadDye.
how the "e.g." relates to the first sentence...

I'd guess it's just a proportion, i.e. 1 part each Live and Dead Dye, then an example showing one part of that mix to 2 parts of the PBS.
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  #1938  
Old 02.04.2020, 22:36
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Re: Ask a Scientist

Ooh. Bored molecular biologist here.


1X means the solution has exactly the correct concentration of "stuff" you need. 2X is a solution that is double concentrated, 1000X is one thousand-fold, etc.



I assume that she begins with a cells in a certain volume of buffer, probably PBS.


Quote:
Label cells with the Live and Dead Dye
Add 1X Live and Dead Dye to cells
(e.g. mix 2X dye in PBS 1:1 with cells in culture media )

In order to have the cells be suspended in a solution containing the correct concentration of Live and Dead Dye she needs to mix her cell suspension with an equal volume of a solution containing the dye at 2X the desired concentration of dye.



Giving an example of a 100 ul sample of cells, and a desired final dye concentration of 10%, if your daughter mixed her cell suspension with 100 ul of a solution containing 20% dye, her final volume would be 200 ul, and the final concentration of dye would be 10% (it was diluted by half).



It seems this Live and Dead dye is initially sold at 1000X concentration, and needs to be diluted from this to make what would be called your 2X working solution.
Quote:
Dilute Live and Dead Dye to 2X concentration in PBS. E.g. 1mL PBS + 2µL 1000X Live and DeadDye.
Technically this should be 2 ul mixed with 998 ul PBS (a 500-fold dilution), but that level of precision isn't necessary with this type of work.
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  #1939  
Old 02.04.2020, 23:03
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Re: Ask a Scientist

Now is the time to hook the next generation EF'ers: Get your descendants on a "please do my home-work" thread.
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  #1940  
Old 03.04.2020, 12:18
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Re: Ask a Scientist

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Monday morning, nothing to do but browse forum threads
Has anyone experience with vaporfly shoes ? Are they as good as the hype? Why are the other companies not coming up with me too products?
If I adapt my old running shoes with a piece of foam and tin plate insert or attach a piece of box-spring mattress to the soles would I be banned from competitive racing?
https://www.wired.com/story/the-scie...marathon-shoe/
I haven't tried them yet, although I'm thinking about it for the autumn, when I hope that we can race again. It's a lot of money and they wear out very fast, so I'll need to be close to world class (for my age) to justify the investment.

It looks like they do work, but it is hard to tell because while almost all the top marathon runners win in them, they are also sponsored by Nike, so they have to wear them. However there is lots of anecdotal evidence of unsponsored runners getting personal bests after switching, as well as the original Nike sponsored (I think) academic study showing the famous 4% improvement.

Competitors haven't issued me-too versions yet due to Nike's patents, I imagine, but they are on the way.

I don't think that you would get a similar improvement with a home made version, but I'd be interested to hear how your trial goes .
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